Molecular Detection of Chicken Anemia Virus from Native Larry-breed Chickens in Chaharmahal-va-Bakhtiyari Province, Iran

Background and Aims: Finding prevalence of chicken anemia virus (CAV) infection in native chickens is necessary to avoid transmission of infection to commercial flocks. In this paper we attempt to describe molecular detection of chicken anemia virus in native Larry-breed chickens in Chaharmahal-va-Bakhtiyari province in Iran for the first time. Materials and Methods: Blood samples were collected from 100 native Larry-breed chickens (5-8 months old) in Shahrekord, Lordegan, Brogen and Koohrang, i.e.four cities of Chaharmahal-va-Bakhtiyari province. To detect CAV, Polymerase chain reaction (PCR) was undertaken on isolated DNA from blood samples using a pair of CAV specific primers that produced a 374 base pair fragment. Results: PCR analysis detected CAV in 12 of 100 (12%) tested blood samples. Conclusions: The results revealed that native Larry chicks were not free from CAV infection in Chaharmahal-va-Bakhtiyari province and vaccination against CAV should be taken into account in native farms.